The Gene Pulser Xcell™ is a modular electroporation system that includes a main unit, two accessory modules (the capacitance extender, or CE module, and . The Gene Pulser Xcell total system is the complete electroporation system for transfection of both eukaryotic and prokaryotic cells. The Gene Pulser Xcell total. We have one Year BIO-RAD Gene Pulser xCell Total Electroporation System for sale. Lab paid $ in Year but had to close.
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Why are Certificates of Analysis not on the Documents tab? Moreover, how will the size of the cuvettes and volume of cell suspension influence the transfection efficiency? Let the cells recover for 24 hours. Please reenter your email gehe in the correct format. There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. I actually have a problem with getting good viability for urine iPSC.
July 31 st1 Reply Post a Pukser. Please check your Internet connection and reload this page. Hello, Thank you for your inquiry.
Outputs and other specifications: Integral leads are included with the PC module. The cells are largely confluent due to the high cell density. Gene Pulser Refine Results. All pictures shown above are of actual product s sold. To receive the latest news, promotions, and more, genee up for Bio-Rad updates by entering your email address below.
Browse Related Browse Related. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product’s expiration date.
Enter your email address below to receive your choice of the latest news, promotions, and more. After transfecting cells and allowing them to recover, analyze the transfection efficiency qualitatively, using epifluorescent microscopy, and quantitatively, using flow cytometry. The high capacitance settings need to be multiplied by to get units of uF, so a setting of 0. This product contains electrical or electronic material.
We are interested in the percentage of cells xecll are GFP positive under each condition and how the percentages vary with cell age. Missing dial knob, but unit works. The PC module is recommended for the electroporation of bacteria and fungi, as well as other applications where high-voltage pulses are applied to samples of small volume and high resistance.
A choice of exponential or square waveforms. Remove supernatant and resuspend cells in a known volume of PBS. The shorter the time constant the faster the decay. Find a Certificate of Analysis. It seems you are interested in using a square wave protocol.
Unable geje load video. Detailed parameters can be found for each of these cell lines, with ;ulser for the preparation of electrocompetent cells in the Gene Pulser Xcell system instructions or at www. If not satisfied, You can return this unit withing 14 days for a full refund minus shipping. Electroporation system for mammals fibloblasts into oocytes.
Item was pulled from a working environment comes with power cord onlyit have some scratches and marks but is fully working. To compare transfection among the four mouse embryonic fibroblast MEF cultures, representing three different passage numbers, perform the following on each flask. Maximum W during short charging periods Operating environment: Aspirate off cell xell media. Cells that have been successfully electroporated and are now expressing the GFP gene appear under epifluorescent microscopy.
See the sample label below for the location of this information. Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile. An unexpected error occurred. There are no threads in Xcelll Laboratories, Inc.
I normally recommend determining the volume and cuvette size you need first, and then optimizing the instrument settings. Please try reloading the page. Under epifluorescence, one can see that the number of GFP expressing cells has increased.
Visit Bio-Rad Laboratories, Inc. The same field of view under epifluorescence shows a number of cells are expressing the GFP marker, but these are only a small percentage of the cells visible in the previous image. Manuals and Resources Note: For best results, we recommend optimizing to identify the best conditions for your experiment. Chemical and Viral Based.
Can I get a Certificate of Analysis even if my item is expired? This page was last updated: Gene Pulser electroporation buffer is recommended for mammalian xfell especially difficult to transfect cells including primary and stem cells.
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