PDF | Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted. PDF | Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to. PDF | In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and mg .
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Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from Gerbera petioles. How to cite this article: Micropropagation selected somaclones Begonia Saintpaulia J. Pakistan Journal of Biological Sciences Volume 11 Gerbera plantlets formed were transferred to soil with The physiological status of the donor is determined by environmental conditions such as temperature, light intensity, day-length and light wavelength. Because genetic variability within the Gerbera genus is relatively limited, breeding potential for new flower colours and patterns as well as resistance to biotic or abiotic stresses is also limited Orlikowska et al.
However, in Gerbera jamesoniicytokinin alone in the medium induces the formation of microshoots. Percentage of callus and root formation per explants produced on MS medium supplemented with different hormones and concentration after 8 weeks in culture formed in these experiments were mainly green. Bigot reported that the addition of g L -1 activated charcoal in the culture medium showed vigorous rooting from excised shoots of Begoniax hiemalis.
Callus is capable of forming adventitious roots.
This technology is being utilized commercially in the ornamentals industry and in other plant production organizations worldwide Chu, ; Huetteman and Preece, ; Mantell et al. Direct organogenesis from mature leaf and petiole explants of Eryngium foetidum L. Principles of Plant Biotechnology. In the present study, propagation of Gerbera jamesonii through tissue culture techniques was done and the factors influencing the growth of this plant were studied.
Survival rate of micro micropropagagion plants that were transferred to soil were investigated Fig. Petioles and leaves obtained from aseptic young plantlets formed from the seedlings were used as source of explants. The present micropropagatoon showed that only petiole explant produced optimum results for shoot formation.
Based on the present study, all combinations of hormones used were able to induce callus. However, white, creamy friable callus was obtained when explants were cultured on MS medium supplemented with 2, 4-D alone. Vardja and Vardja reported that, the addition of 10 mg L -1 BAP in culture medium increased the formation of adventitious buds of Gerbera at initiation and multiplication stage.
Abstract Regeneration potentials in Gerbera jamesonii Bolus ex.
However, regeneration of root was not micorpropagation when explants were cultured on MS medium supplemented with BAP and 2, 4-D. Leaf explants cultured on MS medium supplemented with 1. The most important aspect in plant tissue culture is the capability of cultured cells and tissues to regenerate into complete plants.
Addition of auxins together with cytokinins becomes essential for shoot induction and multiplication depending on the plant type.
In Gerbera jamesoniiBAP was also required for the formation of callus. They are planted outdoors in full sun and useful as cut flowers, pot plant and also bedding plant.
Combination of auxin and cytokinin induces the formation of adventitious shoots and roots. Gerbera jamesonii Bolus ex.
Plant Cell Tissue Organ Culture, Highest numbers of shoots from petiole explants 9. A revised medium for rapid growth and bio assays with tobacco tissue cultures. High efficiency adventitious shoot bud formation and plant regeneration from leaf explants of Dianthus chinensis L.
Regenerated plants were rooted on Murashige and Skoog basal medium. Genotype is one of the most influential factors on the response of gerbera in vitro. Kluwer Academic Publishers, Dordrecht, pp: Plant hormones and type of explants play very important roles in determining regeneration of Gerbera jamesonii in vitro.
The mode of action of thidiazuron: The propagation rates via organogenesis can be much higher than axillary shoot proliferation Chun, Shoot formation was observed when capitulum explants were cultured on MS modified medium supplemented with 2.
Optimilization of Gerbera plantlet production from excised capitulum explants.
Shoot tips are the commonly used explant while adventitious shoot induction from the capitulum is also a popular method. Tissues of explants generally show distinct planes of cell division, various specializations of cells and organization into gebrera structures such as the vascular system.